Effect of Different Medium on in Vitro Shooting and Regeneration of Bacopa Monnieri L. an Important Medicinal Plant

Bacopa monnieri L. is an important medicinal plant of Ayurveda to cure various diseases. It is also considered to have property of memory enhancer. Lots of medicinal importance of this plant attracted the scientist attention towards it. The present work is a comparative study for standardization of growth medium and growth hormone for in vitro culture of Bacopa monnieri L. Three different medium (MS, B5 and SH) was compared. These growth medium was supplemented with different concentration of auxin (NAA, IAA and IBA) and Cytokinin (BAP and KIN) alone and in combination. Best shoot bud induction was achieved on MS medium supplemented with 1.0 mg/ L BAP and 1.0 mg/ L KIN. In other medium shoot bud induction was low. Highest number of shoots and shoot length was also observed in the same medium. Highest root induction was achieved on MS medium supplemented with 0.5 mg/ L and 1.0 mg/ L IBA. Root number and root length was also highest in the same medium. Rooted plantlets were first acclimatized in green house and then successfully transferred to field. Survival rate of this plant was achieved 90%. This in vitro regeneration protocol provides a large scale production of Bacopa monnieri L. which will prevent the natural resources of this plant.