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Molecular Characterization and Species Comparison of Esterases from Slugs Tandoniabudapestensis and Deroceras Reticulatum

Author(s):

P. Swapna , KAKATIYA UNIVERSITY; T. Ravinder Reddy, KAKATIYA UNIVERSITY

Keywords:

ER, AcE, ArE, Esdp, CE, d.reticulatum, t.budapestens

Abstract

Esterases from the six tissues of the slugs tandonia budapestensis and deroceras reticulatum collected from komatipally village were extracted and analyzed by using polyacralamide gel electrophoresis, one substrate(1-naphylacetate) and five inhibitors (pCMB, paraoxon, physostigmine, EDTA, AgNo3)were used. Nine esterase bands were detected in both species. The esterase bands were distributed in six main zones, which could be classified as AcE, ArE, CE, ER, Esdp esterases depending on the inhibitor properties and relative mobility. Esdp and AcE esterases are not noticed in ctenidia of both species of slugs and remaining three types (ArE, ER, CE) of esterase are noticed.ER esterases are not noticed in hepatopancreas of d.reticulatum and tentacles of two slugs species, it is noticed in hepatopancreas of t.buda pestensis. Esdp esterases are not noticed intestine of both species of slugs and it is noticed in mantle of d.reticulatum and foot of t.buda pestensis. Fastest bands are noticed in ctenidia hepatopancreas and tentacles of t.budapestens and ctenidia hepato pancreas of d.reticulatum. Slowest zones are noticed in mantle, foot and intestine of both species.

Other Details

Paper ID: IJSRDV4I120725
Published in: Volume : 4, Issue : 12
Publication Date: 01/03/2017
Page(s): 823-826

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