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Quantum Dot Based Aptamer for Optical Detection of Lead II Ions

Author(s):

Rahul Cherukuri , Osmania University; Prashanth Chintalaputti, Osmania University

Keywords:

FRET, TBA, NSET, EDTA

Abstract

Lead in the environment causes people to have unusually high blood lead levels leading to lead poisoning. Therefore, it is important to detect low levels of lead in the environment to prevent lead poisoning in humans. Therefore, it is important to detect low levels of lead in the environment to prevent lead poisoning in humans. In this paper, experimental studies have been performed to detect lead at the micromolar and nanomolar concentration range. The detection of lead ions at this low level is achieved using a DNA aptamer and quantum dots optically. Pb2+ ions can be detected using a DNA aptamer known as thrombin binding aptamer (TBA), which folds into a G-quadruplex structure when lead is present in the fluids. The conjugation of a quantum dot (QD) and a gold nanoparticle (AuNP) to the TBA allows for optical detection via Fluorescence Resonant Energy Transfer (FRET). In this aptamer probe discussed herein, the excited quantum dot fluoresces with the DNA. Once the Pb2+ ion interacts with TBA, folding of the DNA occurs causing the fluorescence to reduce through energy transfer to the quencher gold nanoparticle. The quenching efficiency observed with the filter paper probe of the handheld device at 10 nM Pb2+ ions is as high as 60%.

Other Details

Paper ID: IJSRDV5I60531
Published in: Volume : 5, Issue : 6
Publication Date: 01/09/2017
Page(s): 1143-1145

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