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Development and Validation of UV Spectrophotometric Method for the Estimation of Some Hepatitis-C Drugs in Bulk and in Formulations

Author(s):

N. Anuradha , H.L.T. College of Pharmacy, Kengal, Channapatna, Karnataka- 562161; Dr. Syed Azhar Nizami, H.L.T. College of Pharmacy, Kengal, Channapatna, Karnataka- 562161

Keywords:

Boceprevir, Simeprevir, Method Development, Validation, & Spectrophotometeric Analysis

Abstract

The present work is to develop a simple, efficient, and reproducible UV spectrophotometeric method for the estimation of some hepatitis-C drugs Boceprevir and Simeprevir in bulk and in tablet dosage form. The developed method is based on measurement of absorption at a wavelength maximum (λmax) of 235 nm for Boceprevir and 225 nm for Simeprevir using methanol as solvent. Linearity was observed in the concentration range 2-12 μg/ml for both the drugs. The results of analysis have been validated statistically for linearity, precision, accuracy, LOD and LOQ as per ICH guidelines. The % assay for the pure sample was found to be 100.33% for Boceprevir and 100.50% for Simeprevir. Recovery studies for the method was found to be 101.02%, 101.35% and 100.18% for Boceprevir and 102.03%, 101.45% and 101.18% for Simeprevir. The developed method was found to be accurate, precise, selective and rapid for spectrophotometeric analysis of Boceprevir and Simeprevir in bulk and in dosage forms.

Other Details

Paper ID: IJSRDV6I80064
Published in: Volume : 6, Issue : 8
Publication Date: 01/11/2018
Page(s): 202-205

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