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Antibacterial Properties of Grewia Asiatica and Cuscuta Reflexa

Author(s):

Pankaj Kumar , Boston College for Professional Studies, Gwalior; Keshav Kumar Choudhary, Boston College for Professional Studies, Gwalior; Swati Goswami, Boston College for Professional Studies, Gwalior; Reena Jain, Boston College for Professional Studies, Gwalior

Keywords:

Grewia asiatica Leaf Methanol Extract; CRSME: Cuscuta reflexa Stem Methanol Extract; CRSAE: Cuscuta reflexa Stem Acetone Extract; GALAE: Grewia asiatica Leaf Acetone Extract

Abstract

It is estimated that more than 25% of modern medicines are directly or indirectly derived from plants. In this context, it is worth mentioning that Indian medicinal plants are considered a vast source of several pharmaceutically active principles and compounds that are commonly used in home remedies against multiple ailments. Among them two Indian native plants, Grewia asiatica commonly known as Phalsa or Falsa belonging to the family Tiliaceae and Cuscuta reflexa belongs to the family Convolvulaceae have enormous traditional uses against various diseases and many bioactive compounds have been isolated from these plants. The entire plant samples were collected in different months of year 2013. Cuscuta reflexa stem and flowers were collected in October and Grewia asiatica leaves, fruits and seeds were collected in June. Extracts of various plants parts were prepared using solvents like water (cold and hot) and organic solvents (methanol, ethyl acetate, acetone). Antimicrobial activities of different extracts were evaluated by the agar well-diffusion method. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal concentration (MBC) of various extracts was estimated against Escherichia coli and Bacillus cereus. To check antimicrobial activity, minimum inhibitory concentrations (MIC) of different extracts were determined against Escherichia coli and Bacillus cereus using the test tube dilution method. All the plant extracts showed varying degree of antibacterial activity against the test organisms. Agar well diffusion assay was used for evaluating the zone of inhibition. GALAE produced maximum zone of inhibition against Gram positive and Gram negative bacteria under taken for the study such as Bacillus cereus (30±2mm), Citrobacter environmental isolates (19.5±2mm) and Staphylococcus aureus (16±2mm) followed by GALME against Escherichia coli (11.5±2mm), Bacillus cereus (11.5±2mm) and Pseudomonas mendocena (10±2 mm). CRSME developed zone of inhibition against Bacillus cereus (6±2 mm) only whereas CRSAE developed zone of inhibition against E. coli environmental isolates and Bacillus cereus of 5±2mm. MIC and MBC of GALME was 100 mg/ml and for GALAE was 250mg/ml. MIC and MBC concentrations against E.coli were 100mg/ml and 250 mg/ml with CRSAE. Gram positive bacteria are usually more sensitive to crude extracts and bioactive constituents because of the specific structure of their cell walls.

Other Details

Paper ID: IJSRDV7I20053
Published in: Volume : 7, Issue : 2
Publication Date: 01/05/2019
Page(s): 51-58

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