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To study of the genetic variations among the Azospirillum lipoferu isolates using randomly amplified polymorphic DNA (RAPD) marker


Kavit Mehta,B. N. Patel , Mehsana Urban Institute of Sciences, Ganpat University, Mehsana, Gujarat, India; Y. B. Dar, Central Salt Marine Chemical Research Institute (CSMCRI), Bhavnagar, Gujarat, India; N. V. Upadhyay, Department of medicinal and aromatic plant, Anand Agriculture University, Anand, Gujarat, India; B. K. Jain, M.G.Science Institute, Navrangpura, Ahmadabad, Gujarat, India; Suresh C. K, University of Agriculture Science, GKVK ,Bangalore, Karnataka, India


arbitrary primer, auxonography, squared Euclidean distance, principle component analysis


Among free-living microorganisms, which can be practically used in agriculture, bacteria from the Azospirillum genus as well as other endophytes are nowadays thought of as the most active component of associative dinitrogen fixation. The investigation was carried out to study the characterization of Azospirillum lipoferu found in the soils of the ten agro-climatic zones which Karnataka, is classified. By using RAPD markers, 75 bands were scored out of which 78.6 % were found to be polymorphic. Statistical analysis of RAPD data enabled the classification of 10 Azospirillum isolates into two major groups. . In this, the cluster analysis based on 75 RAPD bands revealed that the ten A. lipoferu isolates examined clustered at a linkage distance of about 40 units on the dendrogram. There was no correlation between RAPD and geographical origin of isolates.

Other Details

Paper ID: IJSRDV1I2037
Published in: Volume : 1, Issue : 2
Publication Date: 01/05/2013
Page(s): 205-210

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