Exploitation of Vegetable and Fruit Waste for Cellulase and Pectinase Production Using Aspergillus Sp.

Fermentation technology plays a very important role for production of microbial enzymes, antibiotics, single cell proteins, various food products. Use of Microbes as bioreactors for the developmet of the industrially important enzymes from microbial origin include Lipases, Amylases, Proteases, Cellulase and Pectinases. The current work involved for the production pectinase and cellulase enzyme using decaying fruit and vegetable waste. The organisms were isolated from the waste and were cultured onto SDA agar Plate as the isolation is of Fungi. The organism was screened for enzymatic activity using Carboxyl Methyl Cellulose and Pectin agar medium. The result revealed a clear zone of inhibition in the agar plates. Microscopic identification and Morphology study revealed that the Fungi isolated was Aspergillus niger. Aspergillus niger produced significant quantities of enzyme when grown in production medium under submerged condition and incubating for 10 days. The isolate showing maximum activity was identified and subjected to growth parameters optimization in attempt to increase the enzyme production ability of the isolate in larger scale. The activity of pectinase and cellulase production by fungus at various optimum conditions of pH 7 temperature 37℃ were assessed. The enzymes was determined by titrartion method and used for Quantitative estimation of protein was done using Lowry's method at 660nm to know the concentration of protein and antioxidant test were done. Thus the enzyme was used for fruit juice extraction production. The amount of juice extracted in the control samples was less compared to the samples extracted using Pectinase and Cellulase enzyme. Concluded that Aspergillus niger from fruit and vegetable waste has ability to produce pectinase and cellulase and it is highly beneficial, it may consider as a source for production of industrial enzyme.