Construction of Phylogenetic Tree Using Neighbor Joining Method |
Author(s): |
| Rajvir Kaur , GNDEC, Ludhiana, Punjab, India; Karandeep Kaur, GNDEC, Ludhiana, Punjab, India; Gurpreet Kaur, GNDEC, Ludhiana, Punjab, India |
Keywords: |
| phylogenetic tree, neighbor joining, MEGA 6 |
Abstract |
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Phylogeny provides means to reconstruct the history of life by comparing the different species and explains the present diversity of living creature. The neighbor joining (NJ) method is one of the most widely used methods in phylogenetic analysis. This distance based method iteratively cluster taxa until all the taxa form an unrooted tree. The construction starts with the identification of a set of homologous sequences followed by downloading those sequences. The next step includes alignment of the sequences and then evaluating the tree from those aligned sequences. This paper aims to provide step by step description to generate phylogenetic tree with the help of molecular evolutionary genetics analysis (MEGA 6). The whole process is explained with an example of helicobacter Pylori Antigen. The latest version of MEGA can be downloaded from www.megasoftware.net. |
Other Details |
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Paper ID: IJSRDV2I12310 Published in: Volume : 2, Issue : 12 Publication Date: 01/03/2015 Page(s): 671-674 |
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