Kinetic Studies on Planktonic Cell Growth, Biofilm Formation and Antimicrobials Production by a Salt-Tolerant Estuarine Actinobacterial Strain Streptomyces sundarbansensis sp nov.

In this study, effect of initial cultivation temperature on antimicrobials production, planktonic cell growth and biofilm formation by a salt-tolerant estuarine actinobacterial strain Streptomyces sundarbansensis sp nov. was investigated in sucrose based medium at shaken flask level for three different temperatures (25 oC, 30 oC and 35 oC). Peak antimicrobial activity (35 mm) was found at 30oC compared to the peak values of 29.5 mm, 24 mm attained at 25 °C and 35 °C respectively. From the growth curves obtained for both plantonic and biofilm growth, µmax,B (0.043 h-1)and µmax,P (0.063 h-1) were also maximum at 30oC. Product (antimicrobials) concentration increases linearly with total cell growth that comprised of both the planktonic cell growth and the biofilm growth. Luedeking – Piret model satisfactorily described the dependence of antimicrobials productions on total cell growth. Antimicrobials production was found to be strongly growth associated.