Molecular Characterization of Isolated Methyl Parathion Degrading Bacteria and Gene. |
Author(s): |
| Manoj V. Parakhia , Department of Biotechnology, Junagadh Agricultural university, Junagadh; Rukam S. Tomar, Department of Biotechnology, Junagadh Agricultural university, Junagadh; Megha R. Vadukiya, Department of Biotechnology, Junagadh Agricultural university, Junagadh; Visha M. Rathod, Department of Biotechnology, Junagadh Agricultural university, Junagadh; Ashish J. Bhatt, Department of Biotechnology, Junagadh Agricultural university, Junagadh |
Keywords: |
| Methyl Parathion, RAPD, opd/ mpd gene |
Abstract |
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The burning problem in present era is pesticide residue in fruits and foods. present study focus on degradation of pesticide in contest to that total 45 methyl parathion(MP) degrading bacteria were isolated from sludge of MP producing industrial waste. According to screening 21 highly efficient pesticide degraders were selected at 500ppm concentration of standard methyl parathion as a sole carbon source. All isolates were characterized by RAPD and based on the RAPD result all 21 bacterial isolates were grouped in to 2 main clusters with 58% similarity. Characterization of MP degrading gene was carried out by using specific primer. Out of 21 isolates, 14 isolates were containing mpd gene and 4 isolates were containing opd gene while remaining 3 isolates which did not show amplification with opd/ mpd primer so it may contain other unreported gene responsible for methyl parathion degradation. Isolates were identified based on 16s rRNA sequence and one highly efficient bacterium selected for genome characterization. |
Other Details |
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Paper ID: IJSRDV3I100037 Published in: Volume : 3, Issue : 10 Publication Date: 01/01/2016 Page(s): 46-48 |
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