Optimization of Recombinant Cellulase (Cel A) Production in E. Coli using Statistical Modelling |
Author(s): |
| Goutham Kumar. B , Department of Biotechnology,JNT University,Anantapuram; D. V. R. Prasad, Yogivemana University; K. N. Jayaveera, JNTU |
Keywords: |
| CelA, E. coli, Plackett-Burman design |
Abstract |
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There are various methods of optimizing the fermentation medium and fermentation process conditions like factorial design, biological mimicry, Evolutionary operation factorial design, Plackett-Burman design, Central composite design and Response surface methodology. Each method has advantages and disadvantages. In our study, we have chosen Plackett-Burman design, Central composite design and Response surface methodology to optimize the fermentation medium and fermentation process conditions for Cellulase (CelA) production and purification. The modified HCD media has given good Cellulase (CelA) expression (2.8g/L) and solubility (1.9 g/L) and enzyme activity of 7.9 IU/ml was achieved. |
Other Details |
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Paper ID: IJSRDV5I30687 Published in: Volume : 5, Issue : 3 Publication Date: 01/06/2017 Page(s): 923-933 |
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