Molecular Identification of Papaya Leaf Curl Virus and Betasatellite Associated with Tomato Leaf Curl Disease |
Author(s): |
| Megha Mishra , Mody University of Science and Technology; Rakesh Kumar Verma, Mody University of Science and Technology |
Keywords: |
| Tomato Leaf Curl Disease, Papaya Leaf Curl Virus, Betasatellite |
Abstract |
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A sever leaf curl disease was observed in tomato plants (Solanum lycopersicum) in Haryana during the year 2016. Papaya leaf curl virus (PaLCuV) with Papaya leaf curl betasatellite (PaLCuB) was examined in association with the disease by sequence analysis of ~2.7 kb cloned genome, amplified by Rolling Circle Amplification method from the total DNA of a characteristic infected tomato sample. Sequences were analyzed and submitted to the NCBI database under accession number KU 376493 and KU376494, showing the maximum similarity of 93.99% with papaya leaf curl virus and 97.81% with papaya leaf curl betasatellite respectively. Hence, the isolated virus was identified as PaLCuV and PaLCuB. |
Other Details |
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Paper ID: IJSRDV7I80115 Published in: Volume : 7, Issue : 8 Publication Date: 01/11/2019 Page(s): 573-574 |
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