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Development and Validation of HPTLC Method of Fluoxetine Hydrochloride in Bulk and Pharmaceutical Formulation


Akshay Sampat Shinde , Smt. Kashibai Navale College of Pharmacy


HPTLC, Fluoxetine Hydrochloride, Silica gel G60 F254


A simple, accurate, low cost and specific HPTLC method for estimation of Fluoxetine hydrochloride in capsule has been developed. It was performed on Silica gel G60 F254 aluminium foil using Acetone: Methanol in the ratio of 5:4 as mobile phase. The mobile phase containing chamber was saturated for 10 minutes at room temperature. The Rf value of Fluoxetine was found to be 0.12. The plate was scanned and quantified at 226 nm. The calibration curve response was observed between 300-2100 ng. The linear regression data showed good linear relationship of r2 = 0.999. The percent recovery was found to be 99.94 ±1.188. The developed method was validated for its accuracy and precision with suitable parameters.

Other Details

Paper ID: IJSRDV7I90069
Published in: Volume : 7, Issue : 9
Publication Date: 01/12/2019
Page(s): 65-68

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